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A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis by T. Meier (Engli

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Specificaties

Objectstaat
Nieuw: Een nieuw, ongelezen en ongebruikt boek in perfecte staat waarin geen bladzijden ontbreken of ...
ISBN-13
9783034873512
Book Title
A Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
ISBN
9783034873512
Subject Area
Science, Medical
Publication Name
Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Publisher
Springer Basel A&G
Item Length
9.6 in
Subject
Life Sciences / General, Research
Publication Year
2012
Series
Biomethods Ser.
Type
Textbook
Format
Trade Paperback
Language
English
Author
F. Fahrenholz
Item Weight
16 Oz
Item Width
6.7 in
Number of Pages
Xiv, 240 Pages

Over dit product

Product Information

2.1 Introduction ........................................................... 32 2.2 Technical Procedures ................................................... 32 Chemical Synthesis ..................................................... 35 2.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 38 . . . . . . . . . . . . . Chemical Synthesis ..................................................... 38 Binding to anti-CCK antisera and CCK-B receptors .......................... 40 Binding to streptavidin agarose and photoelution ........................... 40 Affinity chromatography ................................................. 41 2.4 Troubleshooting....................................................... 43 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 44 . . . . . . . . . . . . . . . . . Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands ......................... 45 Summary ............................................................. 45 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . 46 . . . . . . . . . . . . . . . . . 3.2 Technical Procedures ................................................... 48 Preparation of a Biotinylated Ligand ...................................... 48 Preparation of Membrane Fraction ........................................ 50 Solubilization of the PACAP Receptor ..................................... 51 Partial Purification of the PACAP Receptor ................................. 52 Affinity Purification of the PACAP Receptor ................................ 54 Final Purification of the PACAP Receptor .................................. 56 Receptor-Binding Assay ................................................. 57 3.3 Results and Discussion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 58 . . . . . . . . . . . . . 3.4 Troubleshooting ....................................................... 61 Impurity in PACAP27-Cys-NH............................................ 61 2 Low specific activity of the PACAP receptor despite a single band ............. 62 Acknowledgments ..................................................... 62 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . 63 . . . . . . . . . . . . . . . . . Photoreactive Biotinylated Peptide Ligands for Affinity Labeling ......... " 65 Summary ............................................................. 65 4.1 Introduction................................................ . . . . . . . .. . 66 . 4.2 Technical Procedures ................................................. " 67 Synthesis of a trifunctional photoactivatable biotinylating reagent ............. 67 Synthesis of photo reactive biotinylated peptide hormones ................... 67 Site-specific incorporation of biotin and photo labels in separate steps ........ 69 Photoaffinity labeling ................................................... 72 4.3 Results and Discussion .................................................. 76 Synthesis of photoactivatable insulins with permanent biotin labels ............ 76 Applications: Insulin .................................................... 78 VI Examples for other applications .......................................... 78 4.4 Troubleshooting....................................................... 79 References . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81 . . . . . . . . . . . . . . . . . . .

Product Identifiers

Publisher
Springer Basel A&G
ISBN-10
3034873514
ISBN-13
9783034873512
eBay Product ID (ePID)
175746367

Product Key Features

Number of Pages
Xiv, 240 Pages
Language
English
Publication Name
Laboratory Guide to Biotin-Labeling in Biomolecule Analysis
Publication Year
2012
Subject
Life Sciences / General, Research
Type
Textbook
Subject Area
Science, Medical
Author
F. Fahrenholz
Series
Biomethods Ser.
Format
Trade Paperback

Dimensions

Item Weight
16 Oz
Item Length
9.6 in
Item Width
6.7 in

Additional Product Features

Intended Audience
Scholarly & Professional
Dewey Edition
20
Number of Volumes
1 Vol.
Illustrated
Yes
Dewey Decimal
574.19/26
Lc Classification Number
Qh301-705
Table of Content
1 An Introduction to Avidin-Biotin Technology and Options for Biotinylation.- 1.1 Introduction.- 1.2 Technical Procedures.- 1.3 Troubleshooting.- Acknowledgments.- References.- 2 Synthesis of Photocleavable Biotinylated Ligands and Application for Affinity Chromatography.- Summary.- 2.1 Introduction.- 2.2 Technical Procedures.- 2.3 Results and Discussion.- 2.4 Troubleshooting.- References.- 3 Purification of the Receptor for Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) using Biotinylated Ligands.- Summary.- 3.1 Introduction.- 3.2 Technical Procedures.- 3.3 Results and Discussion.- 3.4 Troubleshooting.- Acknowledgments.- References.- 4 Photoreactive Biotinylated Peptide Ligands for Affinity Labeling.- Summary.- 4.1 Introduction.- 4.2 Technical Procedures.- 4.3 Results and Discussion.- 4.4 Troubleshooting.- References.- 5 Immunoprecipitation of Biotinylated Cell Surface Proteins.- Summary.- 5.1 Introduction.- 5.2 Technical Procedures.- 5.3 Results and Discussion.- 5.4 Troubleshooting.- Acknowledgments.- References.- 6 Biotinylation and Chemical Cross-Linking of Membrane Associated Molecules.- Summary.- 6.1 Introduction.- 6.2 Technical Procedures.- 6.3 Results and Discussion.- 6.4 Troubleshooting.- Acknowledgments.- References.- 7 Preparation of Biotinylated Lectins and Application in Microtiter Plate Assays and Western Blotting.- Summary.- 7.1 Introduction.- 7.2 Technical Procedures.- 7.3 Results and Discussion.- 7.4 Troubleshooting.- Acknowledgements.- References.- 8 Biotin-Labeling of Poly(ADP-ribose) in Poly(ADP-ribose)-Protein Interactions.- Summary.- 8.1 Introduction.- 8.2 Technical Procedures.- 8.3 Results and Discussion.- 8.4 Troubleshooting.- Acknowledgments.- References.- 9 Preparation and Use of Biotinylated Probes for the Detection and Characterisation of Serine Proteinase and Serine Proteinas Inhibitory Proteins.- Summary.- 9.1 Introduction.- 9.2 Technical Procedures.- 9.3 Results and Discussion.- 9.4 Troubleshooting.- Acknowledgments.- References.- 10 Avidin/Biotin-Mediated Conjugation of Antibodies to Erythrocytes: An Approach for Immunoerythrocyte Exploration in vivo.- Summary.- 10.1 Introduction.- 10.2 Technical Procedures.- 10.3 Results and Discussion.- 10.4 Troubleshooting.- Acknowledgments.- References.- 11 Biotin in vitro Translation: A Nonradioactive Method for the Synthesis of Biotin-Labeled Proteins in a Cell-Free System.- Summary.- 11.1 Introduction.- 11.2 Technical Procedures.- 11.3 Results and Discussion.- 11.4 Troubleshooting.- Acknowledgments.- References.- 12 Nonradioactive Detection of Nucleic Acids with Biotinylated Probes.- Summary.- 12.1 Introduction.- 12.2 Technical procedures.- 12.3 Results and discussion.- 12.4 Troubleshooting.- References.- 13 Biotin-Labeled Riboprobes to Study RNA-Binding Proteins.- Summary.- 13.1 Introduction.- 13.2 Technical Procedures.- 13.3 Results and Discussion.- 13.4 Troubleshooting.- Acknowledgments.- References.- 14 Rapid YAC End Sequencing by Alu-Vector PCR and Biotinylated Primers.- Summary.- 14.1 Introduction.- 14.2 Technical Procedures.- 14.3 Results and Discussion.- 14.4 Troubleshooting.- Acknowledgments.- References.
Copyright Date
1996

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